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Research Article Open Access

In vitro disease burden analysis using green silver nanoparticles

Deepika Gupta1*, Patima Chauhan1,2

1Environmental Sciences, Department of Botany, University of Allahabad, Allahabad, U.P. 211002, India

2Department of Physics, University of Allahabad, Allahabad, U.P. 211002, India

Adv. Mater. Proc., 2017, 2 (2), 113-116

DOI: 10.5185/amp.2017/210

Publication Date (Web):05 February 2017

Copyright © IAAM-VBRI Press

Abstract


Abstract

Biologically manufactured silver nanoparticles are increasingly being used for various sterilization purposes because of its broad spectrum antibacterial activity. There have been relatively few studies on the applicability of silver NPs to control plant diseases. The present study was aimed to investigate the potential of green synthesized silver nanoparticles (GAgNPs) to analyze disease burden in poppy plants affected with Downy mildew (DM) disease caused by fungi Peronospora arborescens for the first time. The GAgNPs was also assayed to determine its antimicrobial potential against bacterial strains. We found that there were some bacterial strains in addition to the fungus which affected the crop yield, by measuring colony forming unit (CFU), caused disease burden on poppy plants. In in vitro examination shows, GAgNPs significantly inhibited bacterial strains even at 10 ppm (least minimum inhibitory concentration (MIC)) then control. Maximum inhibition shows at 100 ppm (most MIC) which is an optimize concentration of GAgNPs. These results suggest that GAgNPs have potential for use as economic, low-dose, potentially non-persistent anti-microbial agents against both DM fungi and the bacterial strains.     Copyright © 2017 VBRI Press.

Keywords


Disease burden, green silver nanoparticles, downy mildew, colony forming unit, serial dilution.