Effect of plasma irradiation on biocompatibility and cell adhesion of polyaniline/chitosan nanocomposites towards Hep G2 and PBMC cells
Ashok Kumar, Rajiv Borah
Volume 1, Issue 2, Page 146-155, Year 2016 | DOI: 10.5185/amp.2016/206
Keywords: Surface modification, biomaterials, plasma irradiation, biocompatibility, cell viability
Abstract: Surface modification of polymeric biomaterials for tissue engineering applications has drawn considerable research interest. In this work, the surface of polyaniline (PAni) nanofibers/chitosan nanocomposites has been modified by plasma irradiation technique to improve its biocompatibility. The average diameter of PAni nanofibers determined by HRTEM is 35.66 nm, whereas FESEM images depict interconnected network of nanofibers dispersed uniformly throughout the chitosan matrix. XRD patterns of PAni/Chitosan nanocomposites after plasma treatment indicate increase in amorphous nature. The alterations in surface morphology after plasma treatment have been confirmed with the help of SEM analysis. The surface chemistry of the samples after plasma treatment has been investigated by means of ATR-FTIR and contact angle measurements. The ATR-FTIR spectra and surface energy measurements show incorporation of polar functional groups after oxygen (O2) and nitrogen (N2) plasma treatment. Preliminary biocompatibility assessments of the plasma treated PAni/Chitosan nanocomposites have been accomplished using Alamar Blue assay with Hep G2 and Primary peripheral blood mononuclear (PBMC) cells. Both assays show maximum enhancement in cell viability for O2 and N2 plasma treated samples, comparing to the pristine one, whereas least cell viability was observed for Ar plasma treated samples. This study depicts that gas plasma treatment can effectively enhance the bioactivity of PAni/Chitosan nanocomposites and can make them attractive for tissue engineering applications. Copyright © 2016 VBRI Press.