Phycofabrication Of Silver Nanoparticles And Their Antibacterial Activity Against Human Pathogens

Harsha Pinjarkar, Swapnil Gaikwad, Avinash P. Ingle, Aniket Gade, Mahendra Rai*

Nanobiotechnology Lab, Department of Biotechnology, SGB Amravati University, Amravati 444602, Maharashtra, India

Adv. Mater. Lett., 2016, 7 (12), pp 1010-1014

DOI: 10.5185/amlett.2016.6269

Publication Date (Web): Oct 12, 2016,


In the present study, the extracellular phycofabrication (synthesis by algae) of silver nanoparticles was demonstrated using algae i.e. Spirogyra sp. recovered from the fresh water. The reduction of silver ions present in the aqueous solution of silver sulphate (Ag2SO4) was done by the cell filtrate of Spirogyra sp. leading to the synthesis of silver nanoparticles. Phycofabrication of silver nanoparticles was confirmed by using characterization tools like UV-Vis spectrophotometer, FTIR, TEM and NTA. The resulting silver nanoparticles were spherical in shape, in the range of 40-80 nm and capped with proteins. Through the experimental studies, it was found that temperature, pH and salt concentration affects the rate of phycofabrication of silver nanoparticles. Antibacterial study of phycofabricated silver nanoparticles was assessed against human pathogenic bacteria. These silver nanoparticles showed better antibacterial activity against gram positive bacteria i.e. Staphylococcus aureus (ATCC-25923) (13 mm) as compared to Gram negative bacteria i.e. Escherichia coli JM-103 (ATCC-39403) (11 mm). This is the first report of synthesis of silver nanoparticles by Spirogyra sp. using Ag2SO4 as a salt. Extracellular phycofabrication of silver nanoparticles by Spirogyra sp. was found be easy, simple and eco-friendly method.


Extracellular, human pathogenic bacteria, phycofabrication, silver nanoparticles, spirogyra.

Current Issue

Advanced Materials Congress Celebrating 10th Years of Establishment

Secure Management of Networked Batteries for Building Integrated Photovoltaics (BIPV) Systems  

The Cause of 100-year Low Carbonated Concrete of the Bridge 

Organic Montmorillonite Intercalated Nano-composites Prevent Post-Surgical Associated Infections

Morphology/tensile Performance Relationship for LLDPE/PP Double Gated Injected Blends

Preparation and Characterization of Nickel Ferrite Nanoparticles via Sol-gel Method

Nitrogen-doped Diamond Nanowire Gas Sensor for the Detection of Methane

Manufacture of Functional Open-cell Al Foams with Recycled Al Scraps using NaCl Ball Space Holder 

Vilsmeier-Haack Transformations under Non Classical Conditions

New Application of Highly Vesicular Basalt from Jabel Isbil Volcano (Dhamar-Rada'a Volcanic Field), Yemen

An Assessment of Tribological Characteristics under different Operating Condition

Functional Bi Coatings as A Perspective Material for Radiation Shields Production against Electron Radiation

Effects of Silica Modified NiFe2O4 on the Dielectric and Electrical Properties of NiFe2O4 filled Poly (methyl methacrylate) Composites

Previous issues
The Journey of a Decade to Advancing Materials Are the Electrospun Polymers Polymeric Fibers? Mechanical and Thermal Properties of Composite Material and Insulation for a Single Walled Tank for Cryogenic Liquids Prediction of Long-Term Behavior for Dynamically Loaded TPU Investigation of Doped Titanium Dioxide in Anatase Phase. Study ab initio using Density Functional Theory Comparison between Single Al2O3 or HfO2 Single Dielectric Layers and their Nanolaminated Systems Preparation of Stable and Optimized Antibody-gold Nanoparticle Conjugates for Point of Care Test Immunoassays Resonance-Based Temperature Sensors using a Wafer Level Vacuum Packaged SOI MEMS Process Integrated System Based on the Hall Sensors Incorporating Compensation of the Distortions The Efficacy of Cinnamomum Tamala as a Potential Antimicrobial Substance against the Multi-Drug Resistant Enterococcus Faecalis from Clinical Isolates The Effect of Complexing Reagent on Structural, Electrical and Optical Properties of CuS Thin Film Laser Cladding of Fluorapatite Nanopowders on Ti6Al4V Preparation and Evaluation of Sulfonate Polyethylene Glycol Borate Ester as a Modifier of Functional Properties of Complex Petroleum Lithium Grease

Upcoming Congress

Knowledge Experience at Sea TM