Ionic Salt Induced Morphology And Drug Release Control Of Insulin Incorporated Biodegradable PLGA Microspheres

Himansu Sekhar Nanda1, 2, 3, Naoki Kawazoe1, Guoping Chen1, 2*

1Tissue Regeneration Materials Group, International Centre for Materials Nanoarchitectonics,National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan

2Department of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8571, Japan

3School of Materials Science and Engineering, Nanyang Technological University, 50 Nanyang Avenue, Singapore 639798, Singapore

Adv. Mater. Lett., 2016, 7 (11), pp 866-871

DOI: 10.5185/amlett.2016.6907

Publication Date (Web): Oct 01, 2016


Biodegradable polymeric microspheres have been used for microencapsulation of number of drugs for controlled delivery applications. Water-in-oil-in-water (w/o/w) double emulsion has been employed for preparation of drug incorporated poly(lactic-co-glycolic acid) (PLGA) microspheres. In the present study, existing double emulsion method was modified by introducing ionic salt in continuous phase of emulsion process. Insulin incorporated microspheres were prepared from wide range of PLGA concentrations under an identical preparation condition and the influence of varied concentration of salt on microsphere characteristics was studied. The results demonstrated, the degree of solidification of PLGA was controlled using ionic salt and the prepared formulations showed improved morphology, enhanced encapsulation efficiency and a positive modulation over the drug release characteristic compared to control. The modified method should be useful for elimination of highly porous and collapsed microspheres in the formulations prepared from low range PLGA concentration and should pave the way to improve several microsphere formulations for controlled drug delivery applications.


Biodegradable polymers, microsphere, double emulsion, ionic salt, insulin.

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