Amity Institute of Nanotechnology, Amity University, Noida 201303, Uttar Pradesh, India
Adv. Mater. Lett., 2016, 7 (8), pp 666-672
DOI: 10.5185/amlett.2016.6177
Publication Date (Web): Jul 01, 2016
Copyright © IAAM-VBRI Press
E-mail: nidhichauhan2007@rediffmail.com
The glucose level measurement in the blood of diabetic patients without significant variations is important. The level of glycated hemoglobin (HbA1c) in the blood provides an authentic tool for glucose level measurement. In our study, a biosensing system established on properly aligned single-crystal zinc oxide (ZnO) nanorods structures grown on indium-tin oxide coated glass plate (ITO) electrode. ZnO nanorods were immobilized with fructosyl amino-acid oxidase (FAO) enzyme through physical adsorption integrated with cross linking molecules N-5-azido-2-nitro-benzoyloxysuccinimide (ANB-NOS). Whole blood samples were first hemolyzed & then properly digested with protease prior to measuring the HbA1c through the sensor. This enzyme biosensor reported an optimum response at +0.2 V. This biosensor displayed a significant sensitivity and detection limit (0.1μM), fast response time (4s) and wide linear range (from 0.1 to 2000 μM). The enzyme/working electrode is stable for about 4 months, when kept at 4 oC. This recommended biosensor method may apply for detecting HbA1c in blood samples obtained from apparently healthy as well as diabetic patients.
Fructosyl amino-acid oxidase, ZnO nanorods, ITO electrode, glycated hemoglobin, whole blood.
Water and Sanitation Management: During and after COVID-19 Pandemic
Polymeric Energy Materials: Development and Challenges
Influence of Different Copolymer Based Compatibilizers on Performance of Pristine and Recycled PP/PE blends
Bio Ceramics: New Material from Mangrove Bark
Hybrid Physical Chemical Vapour Deposition (HPCVD) of Superconducting MgB2 Thin Films on three Dimensional Copper Substrates
Photoinduced Electron Transfer Reactions of tris(4,4’-dimethoxy-2,2’-bipyridyl)ruthenium(II)cation with Quinones in Aqueous Medium
A Comparative Study of Green Synthesized AgNPs using Carissa Carandas and Nerium indicum Leaves and its Activities against Selected Human Pathogens and MCF 7 Cell
Design and Modelling of Carbon Fiber Grid Structure based Carbon/Epoxy Composites for Enhanced Microwave Absorbing Properties
Elaboration and Characterization of Macroporous Bioceramics using Polymeric Sponge Replication Method
Advanced Healthcare Biomaterials
Piezoelectric Alternative Energy Sources as a Part of the Global Energy Concerns – Future Prospects in the Science and Market
Rectify the Injury-Induced Microenvironment Imbalance in Peripheral Nerve Repair
A Mini Review on Biodegradable Magnesium Alloy Vascular Stent
Prospective Applications of Marine Oriented Materials in the Repair of Peripheral Nerve Injury
Design and Implementation of Dynamic FPAA Based Interface Circuit for Thin Film Lead-Free Piezoelectric Sensors
An Аpproach for Nanostructuring of Piezoelectric Materials by Template-assisted Growth in Porous Aluminum Oxide
Study of Sputtered Barium Strontium Titanate Films for Energy Harvesting Applications
The Effect of Bias on Properties of DLC Coatings for Artificial Joints
Fabrication of Tens-micron-sized Functionalized Polystyrene Microspheres for Utilization in Chiral Drug Isolation
Copyright ©2010-2020 VBRI Press
Full Article