A prototype synthesis and characterization of hydroxyapatite bioceramics nanocrystallites A prototype synthesis and characterization of hydroxyapatite bioceramics nanocrystallites
1Department of Chemistry, Y. M. College, Bharati Vidyapeeth Deemed University, Pune 411038, Maharashtra, India
2Metal Oxide Research Laboratory, Department of Chemistry, Dr. Patangrao Kadam Mahavidyalaya, Sangli 416416, Maharashtra, India
3Stem Plus Biotech Pvt. Ltd, Block-11, Shiv Meridian, M G Road, Sangli 416416, Maharashtra, India
4Raja Shripatrao Bhagwantrao Mahavidyalaya, Aundh, Dist-Satara, Maharashtra, India
Adv. Mater. Lett., 2016, 7 (4), pp 325-329
Publication Date (Web): Mar 07, 2016
Copyright © IAAM-VBRI Press
Novel porous bioactive hydroxyapatite (HAP) nanocrystallites were synthesized by auto-combustion technique. The texture properties of the HAP nanopowder were determined through series of characterization techniques. The thermal decomposition behaviour and required sintering temperature of citrate precursor to phase formation of the sample was checked by thermogravimetric analysis (TG-DTA). The hexagonal structure and porous nature of the material were estimated by using X-ray diffraction (XRD) and electron microscopy scanned with different magnifications. Phase formation of HAP was determined by FTIR technique. Transmission electron microscopic (TEM) study indicated the nanostructure of the ceramics, particle size was found to be 30 - 35 nm. These nanoparticles were evaluated for the qualitative and quantitative determination of different elements by energy-dispersive X-ray spectroscopy. In vitro MTT assays showed HAP nanopowder have good biocompatibility and promotes cell proliferation. This nanostructured HAP powder with enhanced biocompatibility can be potentially used as a material for bone tissue engineering.
Nanocrystallites, hydroxyapatite, porous material, MTT assays.