Biosynthesis Of Bimetallic Au-Ag Nanoparticles Using Ocimum Basilicum (L.) With Antidiabetic And Antimicrobial Properties
V. Malapermal1, J.N. Mbatha1, R.M. Gengan2, K. Anand2*
1Department of Biomedical and Clinical Technology, Faculty of Health Science, Durban University of Technology, Durban, 4001, South Africa
Adv. Mater. Lett., 2015, 6 (12), pp 1050-1057
DOI: 10.5185/amlett.2015.5997
Publication Date (Web): Nov 24, 2015
Copyright © 2019 VBRI Press
E-mail: organicanand@gmail.com
This study was aimed at developing a simple, eco-friendly and cost effective green chemistry method for the synthesis of bimetallic Au-Ag nanoparticles using Ocimum basilicum aqueous leaf and flower extracts, respectively as the natural reducing agents. The successive reduction of chloroauric acid and silver nitrate led to the formation of Au-Ag nanoparticles within 10 min at room temperature, suggesting a higher reaction rate than chemical methods involved in the synthesis. Stable, spherical nanoparticles with well-defined dimensions of average size of 3-25 nm was confirmed by UV-Visible spectroscopy, TEM, SEM-EDX, DLS, and zeta potential, whilst, FTIR in combination with GC-MS analyzed the functional groups adhered to the surface of the nanoparticles The colloidal suspension displayed enhanced antihyperglycemic activity at 69.97 ± 3.42% (leaf) against α-amylase (from porcine) and at 85.77 ± 5.82% (flower) against Bacillus stearothermophilus α-glucosidase than that of acarbose and their respective crude extracts. Furthermore, revealed good antibacterial activity against bacterial species Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa.
Ocimum basilicum,bimetallic (Au-Ag) NPs, &alpha, -amylase, &alpha, -glucosidase, diabetes mellitus.
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