Cover Page December-2015-Advanced Materials Letters

Advanced Materials Letters

Volume 6, Issue 12, Pages 1050-1057, December 2015
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Biosynthesis Of Bimetallic Au-Ag Nanoparticles Using Ocimum Basilicum (L.) With Antidiabetic And Antimicrobial Properties

V. Malapermal1, J.N. Mbatha1, R.M. Gengan2, K. Anand2*

1Department of Biomedical and Clinical Technology, Faculty of Health Science, Durban University of Technology, Durban, 4001, South Africa

Adv. Mater. Lett., 2015, 6 (12), pp 1050-1057

DOI: 10.5185/amlett.2015.5997

Publication Date (Web): Nov 24, 2015

E-mail: organicanand@gmail.com

Abstract

This study was aimed at developing a simple, eco-friendly and cost effective green chemistry method for the synthesis of bimetallic Au-Ag nanoparticles using Ocimum basilicum aqueous leaf and flower extracts, respectively as the natural reducing agents. The successive reduction of chloroauric acid and silver nitrate led to the formation of Au-Ag nanoparticles within 10 min at room temperature, suggesting a higher reaction rate than chemical methods involved in the synthesis. Stable, spherical nanoparticles with well-defined dimensions of average size of 3-25 nm was confirmed by UV-Visible spectroscopy, TEM, SEM-EDX, DLS, and zeta potential, whilst, FTIR in combination with GC-MS analyzed the functional groups adhered to the surface of the nanoparticles The colloidal suspension displayed enhanced antihyperglycemic activity at 69.97 ± 3.42% (leaf) against α-amylase (from porcine) and at 85.77 ± 5.82% (flower) against Bacillus stearothermophilus α-glucosidase than that of acarbose and their respective crude extracts. Furthermore, revealed good antibacterial activity against bacterial species Staphylococcus aureus, Escherichia coli, Bacillus subtilis and Pseudomonas aeruginosa.

Keywords

Ocimum basilicum,bimetallic (Au-Ag) NPs, &alpha, -amylase, &alpha, -glucosidase, diabetes mellitus.

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