A Third Generation Bilirubin Sensor Development By Using Gold Nanomaterial As An Immobilization Matrix For Signal Amplification

Jagriti Narang1*, Nidhi Chauhan1, Ashish Mathur1, Vivek Chaturvedi1, C.S. Pundir2

1Amity Institute of Nanotechnology, Amity University, Noida (UP), India

2Department of Biochemistry, M. D. University, Rohtak 124 001, Haryana, India

Adv. Mater. Lett., 2015, 6 (11), pp 1012-1017

DOI: 10.5185/amlett.2015.5990

Publication Date (Web): Oct 26, 2015

E-mail: jags_biotech@yahoo.co.in

Abstract


In present work, we employed gold nanorods for electrochemical sensing of bilirubin. A new method is developed by using covalently immobilized bilirubin oxidase (BOx) on gold nanorods and employed gold microelectrode. The sensing interface materials were characterized by dynamic light scattering (DLS), scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and  cyclic voltammetry (CV). The optimum response was observed at pH 7 and 35 ºC. The linear working range of the biosensor is 0.01 -500 µM.  Fabricated sensing technology showed good evaluation parameters like precision (2.2 % and 3.2 %) and analytical recovery (98.2 % and 97.4 %). Bilirubin biosensor can be employed for early detection of bilirubin in blood serum to check jaundice,hyperbilirubinia and physiologic jaundice in infants.

Keywords

Bilirubin, gold nanorods, horse radish peroxidase, gold microelectrode.

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