Effects Of Infrared And Ultraviolet Radiation On The Viability Of Cells Immobilized In Porous TiNi-based Alloy Scaffold

Sergey Gunther1, Oleg Kokorev1, Timofey Chekalkin1*, Valentina Hodorenko1, Georgiy Dambaev2, Ji-Hoon Kang3, Victor Gunther1

1Research Institute of Medical Materials, Tomsk State University, ul. 19 Gv. divizii, 17, Tomsk 634045, Russia

2Department of Clinical Surgery, Siberian State Medical University, ul Moskovskiy tr, 1, Tomsk 634050, Russia

3Kang & Park Medical Co., O-song Saengmyung1-ro, Chungcheongbukdo, O-Song 363-951, South Korea

Adv. Mater. Lett., 2015, 6 (9), pp 774-778

DOI: 10.5185/amlett.2015.5882

Publication Date (Web): Sep 06, 2015

E-mail: tc77@mail2000.ru

Abstract


Cell responses to electromagnetic radiation are due to many factors including the cellular microenvironment. The aim of the present study was to explore the effects of ultraviolet (UV) and infrared (IR) irradiation of low intensity on cultured cells derived from different biological tissues (spleen, bone marrow, and Ehrlich's adenocarcinoma), which were immobilized in a porous TiNi-based alloy scaffold. Accordingly, the following objectives were set: i) to evaluate the impact of low-intensity radiation on cell suspensions, and ii) to carry out a comparative analysis of the viability of cells immobilized in porous TiNi-based alloy and IR- and UV-irradiated. The data show that the extracellular environment of bone marrow, tumor and spleen cell populations affects their viability and proliferative potency in porous TiNi-based scaffolds. IR- and UV irradiation of cell cultures immobilized in the scaffold affects the cell viability in populations of bone marrow, tumor, and spleen cells. In case of IR irradiation, cell viability was significantly improved, at the same time UV irradiation suppressed cell proliferation activity. The effect of IR irradiation can be used to resuscitate the cell area. The effect of UV irradiation can be used to destroy residual tumor lesions or other pathological cell populations. Effects of low-intensity infrared (IR) and ultraviolet (UV) radiation on the number of viable cells were evaluated against the control group in which cells were exposed to natural daylight. The results showed that IR irradiation led to a 4.6-, 2.5-, and 1.3-fold increase in viable Ehrlich tumor, bone marrow, and spleen cells, respectively, while UV exposure led to a 3.9-, 1.5-, and 1.2-fold increase, respectively.

Keywords

Cell cultures, cell tissue engineering, porous TiNi scaffold, IR and UV radiation.

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