Development Of Lysine Biosensor Based On Core Shell Magnetic Nanoparticle And Multiwalled Carbon nanotube Composite

Jagriti Narang1, Utkarsh Jain2, Nitesh Malhotra1, Sandeep Singh3, Nidhi Chauhan1* 

1Amity Institute of Nanotechnology, Amity University, Noida 201303, Uttar Pradesh, India

2Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, India

3Department of Biochemistry, M. D. University, Rohtak 124001, Haryana, India

Adv. Mater. Lett., 2015, 6 (5), pp 407-413

DOI: 10.5185/amlett.2015.5735

Publication Date (Web): May 05, 2015

E-mail: nidhichauhan2007@rediffmail.com

Abstract


An amperometric lysine biosensor was fabricated by immobilizing lysine oxidase onto core shell magnetic nanoparticles (Core–shell MNPs)/multiwalled carbon nanotube (MWCNT) layer deposited on Au electrode via carbodiimide linkage. Transmission electron microscopy (TEM) for core–shell MNPs, scanning electron microscopy (SEM), electrochemical impedance spectroscopy (EIS) and Fourier transform impedance spectroscopy (FTIR) studies were used to characterize the modified electrode. Sensor showed optimal response within 2s at 30ºC in 0.05 M sodium phosphate buffer pH 6.0 when polarized at +0.2 V vs. Ag/AgCl. Linear working range of the biosensor was determined by 0.05 -700 μM with a detection limit of 0.05 μM. A good correlation (r = 0.98) was obtained between serum lysine levels measured by the standard HPLC method (y) and the present method (x). A number of serum substances had practically no interference. The sensor was used in 150 assays and had a storage life of 180 days at 4oC. This nanohybrid biosensor will be useful for detection of lysine in food and pharmaceutical industries.

Keywords

Lysine oxidase, lysine, core&ndash,shell MNPs, multiwalled carbon nanotube.

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