Amperometric Immuno-sensor For Detection Of Toxin Aflatoxin B1 Based On Polyaniline Probe Modified With Mc-IgGs-a-AFB1 Antibodies
Keisham Radhapyari1,2, Raju Khan1*
1Analytical Chemistry Division, CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam, India
2Central Ground Water Board, North Eastern Region, Ministry of Water Resources, Government of India, Tarun Nagar, Guwahati 781005, Assam, India
Adv. Mater. Lett., 2014, 5 (8), pp 435-440
DOI: 10.5185/amlett.2014.5562
Publication Date (Web): Jul 16, 2014
Copyright © 2019 VBRI Press
E-mail: khan.raju@gmail.com
An amperometric sensor for detection of toxin aflatoxin B1 from aspergillus flavus based on conducting polyaniline probe using monoclonal anti-aflatoxin B1 (Mc-IgGs-a-AFB1) antibodies after activation with 3% Bovine Serum Albumin (BSA) through electrochemical polymerization has been proposed. The electrode was fabricated by immobilizing Mc-IgGs-a-AFB1 antibodies molecules onto electrode surface and characterized by cyclic voltammetry (CV), scanning electron microscopy (SEM) and fourier transform infrared spectroscopic (FT-IR) etc. The proposed amperometric immune-sensor has demonstrated excellent electro-analytical properties relative to Aflatoxin B1 in a linear range from 0.20 to 1.30 AngmL-1 with a relatively low detection limit of 0.059 AngmL-1. The present study will help in improving for quantitative determination of mycotoxins in food samples may provide significant improvements in quality control of food safety through a simple, rapid, and sensitive testing system for agricultural products monitoring.
Immuno-sensor, polyaniline, bovine serum albumin, environmental toxins, aflatoxin B1, electrochemical impedance spectroscopy
Researcher of the Year 2019: Professor Enge Wang – A Legendary Researcher of Atomistic World
Graphene in Russia: The Main Centers, Research Areas, Results
Principles and practices of Si light emitting diodes using dressed photons
Dual Effect of Light Irradiation for Surface Relief Gratings Formation in Se-rich Ge-Se Thin Films
Synthesis, Structural Characterization, Dielectric and Piezoelectric Properties of Multiferroic Double-perovskite Bi2FeMnO6 Ceramics
Anisotropic and Nonlinear Mechanical Properties in Two-dimensional Nanomaterials
Anisotropy of Mechanical Properties of an AZ31 Alloy Prepared by SPD Method
Validation of Advanced Constitutive Models for Accurate FE Modeling of TPU
Alignment of Rods and Flakes using Electric Field
Shape Memory Polymer Composites for Long-Term Exposure to Space Environment
In-soil Biodegradation Behavior of Chitosan-Coated Phosphorylated Starch Films
Adaptive FRP Structures for Exterior Applications
Characterization of Plasma Activated Water for Medical Applications
Current Global Scenario of Electric Vehicles
Review on Detection of Phenol in Water
Investigating the Machinability of Metallic Matrix Composites Reinforced by Carbon Nanotubes: A Review
Photocatalytic ZnO based PES Membranes for AOP Water Treatments under UV and Sunlight
Plasma Activated Water as a Source of Nitrogen for Algae Growth
Digital Light Processing (DLP) 3D Printing of Polyethylene Glycol (PEG) Biopolymer, Commercially available Ultra-High and Tough (UHT) Resin and Maghemite (γ-Fe2O3) Nanoparticles Mixture for Tissue Engineering Scaffold Application
Fabrication of Nanoparticle Embedded Polymeric Microbeads as an Efficient Drug Delivery System
Micro/Nanostructured Papers from Bagasse Pulp Reinforced by Nanofibrillated Cellulose from different Agro-Waste Sources
Structural, Optical and Magnetic Properties of Pristine, (Mn, Al) co-doped ZnO Nanocrystallites Synthesized via co-Precipitation Method
Nanosecond Laser Surface Patterning of Ti6Al4V Bio-alloy for Improved Biological Performance
Synthesis of Cu2O/Ag Composite Nanocubes with Promising Photoluminescence and Photodegradation Activity over Methylene Blue Dye
Locally available Clays of Bangladesh as a Replacement of imported Clays for Ceramic Industries
Green Synthesis and Characterization of Silver Nanoparticles using Cassia auriculata Leaves Extract and Its Efficacy as A Potential Antibacterial and Cytotoxic Effect
_1572929082.jpg)
Copyright ©2010-2019 VBRI Press
Full Article