Electrochemical DNA Biosensor For The Detection Of Sanguinarine In Adulterated Mustard Oil

Ravindra P. Singh1*, Da-Yeon Kang1, Jeong-Woo Choi1,2 *

1Interdisciplinary program of Integrated Biotechnology, Sogang University,1 Sinsoo-Dong, Mapo-Gu, Seoul 121-742, South Korea

2Department of Chemical and Biomolecular Engineering, Sogang University,1 Sinsoo-Dong, Mapo-Gu, Seoul 121-742, South Korea

Adv. Mater. Lett., 2010, 1 (1), pp 48-54

DOI: 10.5185/amlett.2010.3106

Publication Date (Web): Apr 08, 2012

E-mail: rpsnpl69@gmail.com, jwchoi@sogang.ac.kr

Abstract


PANi/ClO4 doped films were developed electrochemically to immobilize DNA as biosensing platform to detect sanguinarine from adulterated mustard oils. The principle of technique was based on the interaction/intercalation of sanguinarine with dsDNA using electrochemical method. Further, it was suggested that sanguinarine intercalates with DNA strands forming complexes, results in the decrease of redox peak currents. In addition, the decrease of the peak current is proportional to the concentration of sanguinarine. The results based on the voltammetric signals decreased in concomitant increase of sanguinarine concentrations due to base pairing in dsDNA. The bioelectrode exhibited the detection limits 2–64 μM. The recovery experiment results found between 89% and 121% from spiked edible mustard oil sources. The correlation found between the current vs. concentration of SA with a correlation coefficient of (r2) of 0.995 at 95% confidence limit. UV–VIS, CV, DPV, AFM, and SEM. characterized the bioelectrodes. Thus, the proposed electrochemical DNA biosensor detected SA and promising for real-time analysis of small molecules of environmental interest.

Keywords

Double stranded deoxyribonucleic acid, polyaniline, biosensor, sanguinarine

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