Studies On The Interaction Of DNA With Vitamin B12 Based On The Immobilization Of DsDNA On Nano-scale Hydroxyapatite Coating

Shandong Provincial Key Laboratory of Fluorine Chemistry and Chemical Materials, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250 022, China

Adv. Mater. Lett., 2010, 1 (1), pp 34-39

DOI: 10.5185/amlett.2010.3104

Publication Date (Web): Apr 08, 2012

Copyright © IAAM-VBRI Press

 E-mail: sdjndxwq@163.com, sdjndb@263.net 

Abstract

Nano-scale hydroxyapatite (HAp) was prepared by combining co-precipitation with microemulsion method, which exhibited strong adsorption for DNA due to its excellent biocompatibility and particular adsorbability. DNA and HAp could be modified onto glassy carbon electrode (GCE) by the simple and convenient “tip-coating” method. Cyclic voltammetry was used to investigate the interaction of DNA immobilized on the HAp film with vitamin B12 (VB₁₂). The existence of DNA led to the decrease of reduction current of VB₁₂. Both the electron transfer coefficient (α) and the standard rate constant (k_s) were different obtained on GCE and dsDNA/HAp/GCE, which indicated the formation of an electrochemical inactive super molecular complex DNA-nVB¹². The equilibrium constant of this complex was calculated to be 5.35 × 10⁵ mol·L^-1 and the binding number between DNA and VB12 of the complex were determined to be one.

Keywords

Vitamin B12, DNA, hydroxyapatite, interaction, cyclic voltammetry