Green Synthesis of ZnO and Ag-ZnO Nanoparticles using Macrotyloma Uniflorum: Evaluation of Antibacterial Activity Green Synthesis of ZnO and Ag-ZnO Nanoparticles using Macrotyloma Uniflorum: Evaluation of Antibacterial Activity

Green Synthesis of ZnO and Ag-ZnO Nanoparticles using Macrotyloma Uniflorum: Evaluation of Antibacterial Activity

Raghavendra K. Sali1,2, Malatesh S. Pujar1, Shivaprasadagouda Patil1,2, Ashok H. Sidarai1,*,

1Department of studies in Physics, Karnatak University Dharwad 580 003, Karnataka, India

2Department of Physics, J S S Arts, Science and Commerce College, Gokak 591 307, Karnataka, India

Adv. Mater. Lett., 2021, 12 (7), 21071645

DOI: 10.5185/amlett.2021.071645

Publication Date (Web): Jun 12, 2021

E-mail: ashok_sidarai@rediffmail.com

Abstract


In this work, using Macrotyloma Uniflorum leaves an affordable and eco-friendly, ZnO and Ag-ZnO metal oxide nanoparticles were reported. Ensuing ZnO and Ag-ZnO nanoparticles were characterized by UV–visible spectroscopy, FT-IR Spectroscopy, X-ray diffraction, Scanning Electron Microscopy with Energy Dispersive Spectroscopy, Transmission Electron Microscopy, and Dynamic Light Scattering. The green synthesized ZnO and Ag-ZnO nanoparticles comprise an average size of about 120.16 nm and 91.17 nm respectively. The minimum inhibitory concentrations (MIC) of these ZnO and Ag-ZnO nanoparticles and mixtures thereof, Ag-ZnO, were determined on B.subtilis, Streptococci and E.coli cultures. MIC and their antimicrobial activity were studied in vitro; both types of nanoparticles showed high antibacterial activity. Also, it has shown excellent results with MIC value of 62.5 µg/ml for antibacterial activity against ZnO and Ag-ZnO nanoparticles. The Ag-ZnO nanoparticles were shown better antimicrobial effect than the ZnO nanoparticles. So, we can strongly suggest these green synthesized nanoparticles as a potent agent for biological applications.

Keywords

Antimicrobial activity, macrotyloma uniflorum, morphology, nanoparticles, MIC.

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