Cover Page December-2010-Advanced Materials Letters

Advanced Materials Letters

Volume 1, Issue 3, Pages 217-224, December 2010
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Voltammetric Detection Of Microcystis Genus Specific-sequence With Disposable Screenprinted Electrode Modified With Gold Nanoparticles

Minbo Lan1,2*, Chen  Chen1,  Qin Zhou1,  Yuanjie Teng1,  Hongli  Zhao1,  Xiangheng Niu1

1Shanghai Key  Laboratory of  Functional Materials Chemistry, and  Research Centre of  Analysis and  Test,  East China

University of Science and Technology, Shanghai 200237, P. R. China

2State Key Laboratory of Bioreactor Engineering. East China University of Science and Technology, Shanghai 200237, P. R. China

Adv. Mater. Lett., 2010, 1 (3), pp 217-224

DOI: 10.5185/amlett.2010.

Publication Date (Web): Apr 08, 2012

E-mail: minbolan@ecust.edu.cn

Abstract

A disposable electrochemical biosensor for detection of DNA sequence related to Microcystis was described. After depositing gold nanoparticles (AuNPs) onto a screen-printed carbon electrode (SPCE) surface by electrodeposition, the DNA probe which is complementary to a specific gene sequence related to Microcystis was immobilized onto the AuNPs modified SPCE via a thiol linker at the 5’ end. On the electrode surface the immobilized single-stranded DNA could undergo hybridization in the solution containing DNA specific-sequence of Microcystis. Using methylene blue as the indicator, DNA immobilization and hybridization were characterized by cyclic voltammetry and differential pulse voltammetry. Parameters, such as the deposition conditions of gold nanopariticle and the preconcentration time of MB, were optimized. In addition, the control experiments with non-complementary and single base mismatch sequences were investigated and results demonstrated the high selectivity of the biosensor.

Keywords

Microcystis, water bloom, gold nanoparticles,screen printed carbon electrode

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