TiO2 nanoparticles induced oxidative stress mediated DNA damage in the liver of adult male Wistar rats

Dasal Vasantharaja1*, Venugopal Ramalingam1, Shanmugam Thangapandiyan2, Nagarajan Sridhar2, Gaddam Dayanand Reddy3 

1Department of Zoology, Kanchi Mamunivar Centre for Post Graduate Studies (Pondicherry University), Lawspet, Puducherry 605008, India

2Department of Zoology, Annamalai University, Annamalai Nagar 608002, Tamil Nadu, India

3Department of Pharmacology, Siddha Central Research Institute, Chennai 600106, India

Adv. Mater. Lett., 2019, 10 (2), pp 145-150

DOI: 10.5185/amlett.2019.1532

Publication Date (Web): Dec 19, 2018

E-mail: vasaini20@gmail.com

Abstract


Nanoparticles (NPs) are extensively being used in modern life due to their distinctive properties like small size having large surface area. Titanium dioxide (TiO2) NPs are mostly used in cosmetic products, food additives, pharmaceuticals and electronics. They are capable of inducing oxidative stress in both animals and human. The intention of this study was to find out the hepatotoxic effect of TiO2 NPs on the male Wistar rats. The animals were divided in to three groups. First group received normal saline; Second group received TiO2 NPs (50 mg/kg/bw) for 14 days continuously, while third group received TiO2 NPs (100 mg/kg/bw) for the same duration. The increased levels of specific markers AST, ALT, ALP, LDH and GGT along with the TBARS, LOOH, CD and PC in the liver clearly shows the hepatotoxic action of TiO2. The hepatotoxic nature of TiO2 NPs was more evidenced by the diminished activity of antioxidant enzymes levels and also showed augmented DNA damage and fragmentation in hepatocytes. In conclusion, the data indicated that TiO2 NPs induced oxidative stress which produces hepatotoxicity in the rat liver.

Keywords

TiO2 NPs, rat liver, ROS, DNA damage, hepatotoxicity.

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