Facile Electrostatic Immobilization of Glucose Oxidase onto Citrate Capped Gold Nanoparticles for Surface-enhanced (Bio)-Catalysis

Ayushi Tiwari1 and Anshuman Mishra2,*

1IB Chemistry, Katedralskolan, Platensgatan 20, Linköping 58 220, Sweden

2Institute of Advanced Materials, IAAM, Teknikringen 4A, Mjärdevi Science Park, 583 30 Linköping, Sweden

Adv. Mater. Lett., 2018, 9 (11), pp 823-830

DOI: 10.5185/amlett.2018.2007

Publication Date (Web): Jul 25, 2018

E-mail: anshuindia@gmail.com

Abstract


Diabetes mellitus is a serious life-time health issue which has been increasing among the greater population, approximately 285 million people carrying this disease worldwide. In this study, we have functionalised the gold nanoparticles (AuNPs) with biocatalytic enabled optical property, it is the subject of the study for detecting glucose towards the development of photometric nano-transducer. The citrate capped AuNPs were used to warrant the electrostatic self-assembly of glucose oxidase (GOx) in the colloidal state. Glucose biocatalysis was studied through the nano-optical function of glucose on the surface of AuNPs. Using surface plasmonic resonance as analytical technique, we have determined the molecular binding interaction between glucose molecule and AuNPs surface. Based on the visible spectrum, successful immobilization of GOx onto AuNPs was demonstrated. The GOx functionalized AuNP exhibits catalytic activities for the oxidation of glucose and resulting change in the absorption peak of colloidal bio-assembly. It was observed that the absorbance at 520 nm was proportional to the concentration of glucose in the test samples. The Lambert-Beer law expresses the linear relationship between the absorbance and glucose concentration at a fixed wavelength, i.e., λmax at 520 nm. The precise detection of glucose is essential to monitor the biological level of glucose in the body. It can be concluded that the nano-(bio) gold surface exhibits a rapid photometric response with changes of glucose concentration in the test samples.

Keywords

Gold nanoparticle, glucose oxidase, self-assembly, photometric analysis, biocatalysis.

Previous issues

Celebrating 10th Years of Diamond Open Access Publishing in Advanced Materials  

Cerebral Oxygenation Studies Through Near Infrared Spectroscopy: A Review

Analysis of Fine Sulfoaluminate Cement by Strength and Thermogravimetric Analysis

Characterization of the Interfacial Surface Energy for Composite Electrical Conduction Measurements using Two Full Range Percolation Threshold Models

Quantitative Detect of Fatigue of Membrane of Erythrocyte in Uniform Shear Field

Ecofriendly-developed Polyacrylic Acid-coated Magnetic Nanoparticles as Catalysts in Photo-fenton Processes

Evaluation of Drug Interactions with Medications Prescribed to Ambulatory Patients with Metabolic Syndrome in Urban Area

Fiber-reinforced Cementitious Composite: Sensitivity Analysis and Parameter Identification 

Evaluation of Drug Utilization Patterns Based on World Health Organization Drug use Indicators at Outpatients Clinics

Are Quantitatively Micro-machined Scaffolds Effective for Cell Technology?

Synthesis and Characterization of Gold Nanoparticles from Lobelia Nicotianifolia Leaf Extract and its Biological Activities

Advanced Oxidations of Tartrazine Azo-dye

Antifungal Activity of Salvia jordanii Against the Oral Thrush Caused by the Cosmopolitan Yeast Candida albicans among Elderly Diabetic Type 2 patients

Upcoming Congress

Knowledge Experience at Sea TM