Efficient siRNA delivery using osmotically active and biodegradable poly(ester amine)  

¹Department of Molecular Genetics, School of Dentistry, Seoul National University, Seoul 08826 Republic of Korea

²Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Aurangabad, MH State, India

³Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, Republic of Korea 

Adv. Mater. Lett., 2018, 9 (8), pp 590-593

DOI: 10.5185/amlett.2018.2026

Publication Date (Web): Jun 14, 2018

Copyright © IAAM-VBRI Press

E-mail: rohi06@snu.ac.kr

Abstract

Biodegradable and hyperbranched poly(ester amine) (PEA) was prepared by reaction of glycerol dimethacrylate (GDM) with  low molecular weight polyethylenimine (LMW-PEI) by Michael addition reaction. This novel gene carrier showed excellent physicochemical properties and relatively low cytotoxicity compared with PEI 25K. It showed excellent transfection efficiency and siRNA delivery. The higher silencing efficiency of PEAs could be attributed to the synergistic effect arising from hyperosmotic glycerol and proton sponge active PEI residues in the PEA backbone.

Keywords

Poly (ester amine), hyperosmotic effect, proton sponge effect, siRNA delivery.