1Research and Technology Center for Biomolecules, Ferdowsi University of Mashhad, Mashhad, Iran
2Department of Food Nanotechnology, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran
3Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
4Department of Biology, School of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
5Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Adv. Mater. Lett., 2018, 9 (5), pp 369-374
Publication Date (Web): May 17, 2018
Copyright © IAAM-VBRI Press
Escherichia coli O157:H7 (E.coli O157:H7), is an extremely infectious and potentially fatal water and food-borne pathogen, responsible for numerous hospitalisations and deaths all over the world. Herein we reported the development of simple and label-free electrochemical aptasensor based on single wall carbon nanotube (SWCNT) modified screen printed electrode (SPE) to detect E.coli O157:H7 bacteria. The electrochemical probe of methylene blue (MB) was used to investigate the interactions between aptamer and E.coli O157:H7. The resulting MB/Apt/SWNT/SPE layer exhibited good current response to detect E.coli O157:H7. The aptasensor detect E.coli O157:H7 in the concentration range of 1.7×101 - 1.1×107 CFU mL-1 and limit of quantification of 1.7×101 CFU mL-1. The proposed aptasensor is rapid, convenient and low-cost for effective sensing of E.coli O157:H7 with high selectivity over interference bacteria. The suitability of the aptasensor for real sample measurements was investigated by recovery studies in tap water sample.
Escherichia coli O157:H7, aptamer, electrochemical aptasensor, label-free detection.