A sensitive electrochemical aptasensor based on single wall carbon nanotube modified screen printed electrode for detection of Escherichia coli O157:H7 A sensitive electrochemical aptasensor based on single wall carbon nanotube modified screen printed electrode for detection of Escherichia coli O157:H7
1Research and Technology Center for Biomolecules, Ferdowsi University of Mashhad, Mashhad, Iran
2Department of Food Nanotechnology, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran
3Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran
4Department of Biology, School of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran
5Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
Adv. Mater. Lett., 2018, 9 (5), pp 369-374
DOI: 10.5185/amlett.2018.1701
Publication Date (Web): May 17, 2018
Copyright © IAAM-VBRI Press
E-mail: elhamsheikhzadeh@yahoo.com
Escherichia coli O157:H7 (E.coli O157:H7), is an extremely infectious and potentially fatal water and food-borne pathogen, responsible for numerous hospitalisations and deaths all over the world. Herein we reported the development of simple and label-free electrochemical aptasensor based on single wall carbon nanotube (SWCNT) modified screen printed electrode (SPE) to detect E.coli O157:H7 bacteria. The electrochemical probe of methylene blue (MB) was used to investigate the interactions between aptamer and E.coli O157:H7. The resulting MB/Apt/SWNT/SPE layer exhibited good current response to detect E.coli O157:H7. The aptasensor detect E.coli O157:H7 in the concentration range of 1.7×101 - 1.1×107 CFU mL-1 and limit of quantification of 1.7×101 CFU mL-1. The proposed aptasensor is rapid, convenient and low-cost for effective sensing of E.coli O157:H7 with high selectivity over interference bacteria. The suitability of the aptasensor for real sample measurements was investigated by recovery studies in tap water sample.
Escherichia coli O157:H7, aptamer, electrochemical aptasensor, label-free detection.
Advanced Materials Research and Innovation Priorities for Accomplishing the Sustainable Development Goals
Contemporary Advances in Humidity Sensing Materials, Methods, and Performances
Roles of the Debye Length and Skin Depth in the Characterization of Space Charge Interactions in Semiconductor Nanoparticles
Antifungal Activity of Iron-gold and Cobalt-gold co-doped ZnO Nanoparticles
Adsorption and Thermodynamic Parameters of Activated Carbon-Diazepam Systems in Simulated Gastric Fluid
Synthesis, Impedance and Current-Voltage Spectroscopic Characterization of Novel Gadolinium Titanate Nano Structures
Removal of Toxic Dyes from Industrial Waste Water using Chitosan Grafted Itaconic Acid Nanocomposites
Structural, Optical and dielectric properties of Sr doped LaVO4
Design, 3D Development and Finite Element Analysis of Cylindrical Mesh Cage Bioimplants from Biometals
Advancements in Materials Technology for Accomplishing Sustainable Development Goals
A Review for Luminescence Property of Materials, Its Detection and Probabilities for Embedding of Luminescence with MEMS Technology
A Review on Low-Cost Adsorbents for Cadmium Pollutant Removal from Industrial Effluents
Study of Applications of Radial Basis Function Network in Forecasting
Simulation of LPG Pressure Vessel under Fire Engulfment
Strength Analysis of Friction Stir Welding (FSW) Joint Under Minimize Rotation Speed of FSW Tool
Formation of Pure In2O3 pallets for OLED Application
Study of Soil Nitrogen Level and Splitting on Nutrient Uptake around the Industries of Chhattisgarh, India