A sensitive electrochemical aptasensor based on single wall carbon nanotube modified screen printed electrode for detection of Escherichia coli O157:H7

M.R. Housaindokht1*, A.Verdian2, E. Sheikhzadeh3, P. Pordeli4, Z. Rouhbakhsh Zaeri3, F. Janati-Fard3, M. Nosrati3 M. Mashreghi4, A. R.Haghparast1, A. Nakhaeipour3, A. A. Esmaeili1, S. Solimani5

1Research and Technology Center for Biomolecules, Ferdowsi University of Mashhad, Mashhad, Iran

2Department of Food Nanotechnology, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran

3Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

4Department of Biology, School of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

5Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran 

Adv. Mater. Lett., 2018, 9 (5), pp 369-374

DOI: 10.5185/amlett.2018.1701

Publication Date (Web): May 17, 2018

E-mail: elhamsheikhzadeh@yahoo.com

Abstract


Escherichia coli O157:H7 (E.coli O157:H7), is an extremely infectious and potentially fatal water and food-borne pathogen, responsible for numerous hospitalisations and deaths all over the world. Herein we reported the development of simple and label-free electrochemical aptasensor based on single wall carbon nanotube (SWCNT) modified screen printed electrode (SPE) to detect E.coli O157:H7 bacteria. The electrochemical probe of methylene blue (MB) was used to investigate the interactions between aptamer and E.coli O157:H7. The resulting MB/Apt/SWNT/SPE layer exhibited good current response to detect E.coli O157:H7. The aptasensor detect E.coli O157:H7 in the concentration range of 1.7×101 - 1.1×107 CFU mL-1 and limit of quantification of 1.7×101 CFU mL-1. The proposed aptasensor is rapid, convenient and low-cost for effective sensing of E.coli O157:H7 with high selectivity over interference bacteria. The suitability of the aptasensor for real sample measurements was investigated by recovery studies in tap water sample. 

Keywords

Escherichia coli O157:H7, aptamer, electrochemical aptasensor, label-free detection.

Current Issue

Advanced Materials Congress Celebrating 10th Years of Establishment


Secure Management of Networked Batteries for Building Integrated Photovoltaics (BIPV) Systems  


The Cause of 100-year Low Carbonated Concrete of the Bridge 


Organic Montmorillonite Intercalated Nano-composites Prevent Post-Surgical Associated Infections


Morphology/tensile Performance Relationship for LLDPE/PP Double Gated Injected Blends


Preparation and Characterization of Nickel Ferrite Nanoparticles via Sol-gel Method


Nitrogen-doped Diamond Nanowire Gas Sensor for the Detection of Methane


Manufacture of Functional Open-cell Al Foams with Recycled Al Scraps using NaCl Ball Space Holder 


Vilsmeier-Haack Transformations under Non Classical Conditions


New Application of Highly Vesicular Basalt from Jabel Isbil Volcano (Dhamar-Rada'a Volcanic Field), Yemen


An Assessment of Tribological Characteristics under different Operating Condition


Functional Bi Coatings as A Perspective Material for Radiation Shields Production against Electron Radiation


Effects of Silica Modified NiFe2O4 on the Dielectric and Electrical Properties of NiFe2O4 filled Poly (methyl methacrylate) Composites


Previous issues
The Journey of a Decade to Advancing Materials Are the Electrospun Polymers Polymeric Fibers? Mechanical and Thermal Properties of Composite Material and Insulation for a Single Walled Tank for Cryogenic Liquids Prediction of Long-Term Behavior for Dynamically Loaded TPU Investigation of Doped Titanium Dioxide in Anatase Phase. Study ab initio using Density Functional Theory Comparison between Single Al2O3 or HfO2 Single Dielectric Layers and their Nanolaminated Systems Preparation of Stable and Optimized Antibody-gold Nanoparticle Conjugates for Point of Care Test Immunoassays Resonance-Based Temperature Sensors using a Wafer Level Vacuum Packaged SOI MEMS Process Integrated System Based on the Hall Sensors Incorporating Compensation of the Distortions The Efficacy of Cinnamomum Tamala as a Potential Antimicrobial Substance against the Multi-Drug Resistant Enterococcus Faecalis from Clinical Isolates The Effect of Complexing Reagent on Structural, Electrical and Optical Properties of CuS Thin Film Laser Cladding of Fluorapatite Nanopowders on Ti6Al4V Preparation and Evaluation of Sulfonate Polyethylene Glycol Borate Ester as a Modifier of Functional Properties of Complex Petroleum Lithium Grease

Upcoming Congress

Knowledge Experience at Sea TM