1Department of Chemistry and Research Centre, N.M.K.R.V. College for Women, Jayanagar, Bangalore 560 011, India
2Department of PG Studies and Research in Biochemistry, Jnana Sahyadri, Kuvempu University,
Shankaraghatta 577 451, Shivamogga, Karnataka, India
3Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, India
Adv. Mater. Lett., 2017, 8 (11), pp 1020-1028
DOI: 10.5185/amlett.2017.1554
Publication Date (Web): Aug 05, 2017
Copyright © IAAM-VBRI Press
E-mail: sureshssmrv@yahoo.co.in, jsmelo@barc.gov.in, neelgund@gmail.com
In this report, extractions of the Calotropis procera polyphenols have been carried out in conventional soxhlet assisted extraction (SAE) and it is compared with microwave assisted extraction (MAE). As an extension of our previous work reported, we attempted to study the electrochemical interaction of the aqueous plant extract with DNA modified electrode Gr/PAH/NT-G/PPy/DNA. The binding constant Kf determined using UV-Vis spectroscopy was found to be 4.91×10-1 and 4.55×10-1 µmol dm-3 for soxhlet assisted plant extract (SAPE) and microwave assisted plant extract (MAPE) respectively. This was consistent with the electrochemical determination of Kf 4.31×10-6 and 4.71×10-4 µmol dm-3 for SAPE and MAPE respectively. Further the surface coverage area (Γs) of the electrode fabricated using DNA was calculated using Chronocoulometry for the plant extracts. The linear range of the aqueous extract on the Gr/PAH/NT-G/PPy/DNA modified electrode was found to be from 1-250 µmol dm-3, with a limit of detection of 0.22 and 0.29 µmol dm-3 mL-1 for SAPE and MAPE respectively. The limit of quantification was found to be 0.68 and 0.88 µmol dm-3 mL-1 for SAPE and MAPE respectively. As a result Calotropis procera can be studied further to applied for anticancer activity. Moreover, electrochemical methods can be a promising technique in drug analysis.
Impedimetric detection, soxhlet assisted extraction, microwave assisted extraction, chronocoulometry, spectrophotometry.
Advancing Materials towards Climate Neutrality by 2050
Efficiency of Nanomaterials for Electrochemical Diagnostics based Point-of-Care Detection of Non-Invasive Oral Cancer Biomarkers
Hybrid Membrane based on Polymer-doped Phosposilicate and their Characterization
A DFT Study of Interaction of (CdSe)3 Quantum Dots with Nucleobases
Low Resistance and High Electromigration Lifetime of Cu-To-Cu Joints Using (111)-Oriented Nanotwinned Copper
Electrochemical Hydrogen Evolution Reaction Triggered by Co2xMo1-xS2 (x = 0. 0.05 and 0.1) in Acidic Medium
Effect of La Dopant in Modified Bismuth Ferrite Ceramics
Biogenic Capped Silver Nanoparticles in Lablal Purpureus Pod Extract Exhibit Selective Antibacterial and Synergistic Anticancer Activity
Transforming Paradigm of Artificial Intelligence for Scalable Clinical Healthcare
A Review on Harnessing Nanomaterials as Promising Materials Interface
A New Epidemic Spreading Model to Predict the Spread and Accumulation of Corona Virus (COVID-19) Positive Cases as a Function of Time
Green Synthesis of ZnO and Ag-ZnO Nanoparticles using Macrotyloma Uniflorum: Evaluation of Antibacterial Activity
Feature Behavior of Resistivity in Bi Foils Obtained by a Melt Spinning Method
Thermomagnetic Properties of Metal and Metal-oxide Nanoparticles
Effect on Structural, Electrical and Temperature Sensing behavior of Neodymium Doped Bismuth Ferrite
Novel Green Chemistry Synthesis of Nano-Hydroxyapatite using Soya Milk as a Natural Stabiliser
Copyright ©2010-2021 VBRI Press
Full Article