1Department of Chemistry and Research Centre, N.M.K.R.V. College for Women, Jayanagar, Bangalore 560 011, India
2Department of PG Studies and Research in Biochemistry, Jnana Sahyadri, Kuvempu University,
Shankaraghatta 577 451, Shivamogga, Karnataka, India
3Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, India
Adv. Mater. Lett., 2017, 8 (11), pp 1020-1028
DOI: 10.5185/amlett.2017.1554
Publication Date (Web): Aug 05, 2017
Copyright © IAAM-VBRI Press
E-mail: sureshssmrv@yahoo.co.in, jsmelo@barc.gov.in, neelgund@gmail.com
In this report, extractions of the Calotropis procera polyphenols have been carried out in conventional soxhlet assisted extraction (SAE) and it is compared with microwave assisted extraction (MAE). As an extension of our previous work reported, we attempted to study the electrochemical interaction of the aqueous plant extract with DNA modified electrode Gr/PAH/NT-G/PPy/DNA. The binding constant Kf determined using UV-Vis spectroscopy was found to be 4.91×10-1 and 4.55×10-1 µmol dm-3 for soxhlet assisted plant extract (SAPE) and microwave assisted plant extract (MAPE) respectively. This was consistent with the electrochemical determination of Kf 4.31×10-6 and 4.71×10-4 µmol dm-3 for SAPE and MAPE respectively. Further the surface coverage area (Γs) of the electrode fabricated using DNA was calculated using Chronocoulometry for the plant extracts. The linear range of the aqueous extract on the Gr/PAH/NT-G/PPy/DNA modified electrode was found to be from 1-250 µmol dm-3, with a limit of detection of 0.22 and 0.29 µmol dm-3 mL-1 for SAPE and MAPE respectively. The limit of quantification was found to be 0.68 and 0.88 µmol dm-3 mL-1 for SAPE and MAPE respectively. As a result Calotropis procera can be studied further to applied for anticancer activity. Moreover, electrochemical methods can be a promising technique in drug analysis.
Impedimetric detection, soxhlet assisted extraction, microwave assisted extraction, chronocoulometry, spectrophotometry.
Perspectives on Published Energy Sources and Smart Energy Supplies
State of Art Review on Nanobubbles
Eggshell Membrane Assisted CdS Nanoparticles for Manganese Removal in Water Treatment
Green Chemistry Synthesis of Nano-Hydroxyapatite using Natural Stabilisers
Structural, Optical and Electrical Properties of CaSnO3 and Ca0.98Nd0.02Sn0.98Ti0.02O3 Synthesized using Sol-Gel Method
Phase Change Materials Reinforced with Aluminium Foam for Latent Heat Storage
Influence of Manganese and Copper Doping on Structural and Optical Properties of Chromium Oxide Nanoparticles
Understanding of intriguing metal to semiconductor transition in Ni0.5Zn0.5Fe2O4 nanoparticulates
Flaw Resistance and Mode - I Fracture Energy Redistribution in Bamboo - A Correlation
COVID-19: A Significant Revival Approach - Concurrent Case Study in India
Recent Advances in Biochar Modification for Energy Storage in Supercapacitors: A Review
Viral Evolution of Multiple Coronavirus Genomes on Genomic Index Maps
Solution Combustion Synthesis and Energy Transfer in LaMgAl11O19:Tb3+/Sm3+ Tunable Phosphor
Influence of Fe3O4 and CTABr on the Rate of Degradation of Methylene Blue by H2O2
Effect of Sulfurization Temperature on RF Sputtered MoS2 Thin Film
Fatigue Analysis for Fe-34.5Mn-l0Al-0.76C Tidal Turbine Blades using Rainflow Algorithm
Modelling for the Study of Thermoelastic Properties of Nanoparticles
Potato Starch Edible Films as Environmentally Friendly Carriers for Model Drug: In vitro Release Study