1Institute for Regenerative Engineering, Uconn Health, Farmington, CT 06030, USA
2Department of Orthopaedic Surgery, Uconn Health, Farmington, CT 06030, USA
3Raymond and Beverly Sackler Center for Biological, Physical and Engineering Sciences, Uconn Health, Farmington, CT 06030, USA
4Department of Material Science and Engineering, University of Connecticut, Storrs, CT 06269, USA
5Department of Biomedical Engineering, University of Connecticut, Storrs, CT 06269, USA
6Department of Chemical and Biomolecular Engineering, University of Connecticut, Storrs, CT 06269, USA
Adv. Mater. Lett., 2017, 8 (5), pp 587-591
DOI: 10.5185/amlett.2017.7106
Publication Date (Web): Apr 04, 2017
Copyright © IAAM-VBRI Press
E-mail: laurencin@uchc.edu
Conducting polymers are emerging as highly attractive materials since they can be used alone or in combination with other biomaterials to provide electrical stimulus for tissue regeneration. Here, we report the fabrication of a novel stimuli-responsive conducting polymer scaffold, which can be used to regulate muscle cell adhesion, proliferation and differentiation. Our goal in this study was to develop electroconductive nanofiber polymer scaffolds that can modulate the cellular physical microenvironment to increase electrical communication between cells and ultimately generate a more robust and functional construct for muscle regeneration. Matrices such as those designed here could have a significant impact in the clinical setting, where muscle atrophy and fatty infiltration prevent healing of common injuries such as rotator cuff tears. The bio-interface consists of a conducting polymer, poly (3,4ethylenedioxythiophene): poly (styrenesulfonate) (PEDOT: PSS), with a dopamine-polymerized biodegradable substrate made from poly (ε-carprolactone) (PCL) that is rationally assembled together based on the native structure of muscle fibers. XPS analysis confirmed that poly (dopamine) deposition on the PCL scaffolds was successful. The coating of PEDOT: PSS on the poly(dopamine) modified PCL scaffolds was stable as both representative peaks were shown. C2C12 cells, a myoblast cell line was cultured on conductive substrates with different concentrations. Biocompatibility and cellular proliferation of the conducting polymer scaffolds were assessed. It was found that conducting polymers scaffolds of all groups were biocompatible. PEDOT:PSS coating of a low and medium concentration(1% and 10%) showed stimulatory effect on C2C12 growth compared to the control groups. These results showed that the presence of PEDOT:PSS at optimum concentration might enhance C2C12 cell growth and proliferation. These conducting polymer scaffolds hold great promise as biomimetic platforms for skeletal muscle regeneration.
Conducting polymers, muscle tissue regeneration, intrinsic conductivity, bioelectronics.
Perspectives on Published Energy Sources and Smart Energy Supplies
State of Art Review on Nanobubbles
Eggshell Membrane Assisted CdS Nanoparticles for Manganese Removal in Water Treatment
Green Chemistry Synthesis of Nano-Hydroxyapatite using Natural Stabilisers
Structural, Optical and Electrical Properties of CaSnO3 and Ca0.98Nd0.02Sn0.98Ti0.02O3 Synthesized using Sol-Gel Method
Phase Change Materials Reinforced with Aluminium Foam for Latent Heat Storage
Influence of Manganese and Copper Doping on Structural and Optical Properties of Chromium Oxide Nanoparticles
Understanding of intriguing metal to semiconductor transition in Ni0.5Zn0.5Fe2O4 nanoparticulates
Flaw Resistance and Mode - I Fracture Energy Redistribution in Bamboo - A Correlation
COVID-19: A Significant Revival Approach - Concurrent Case Study in India
Recent Advances in Biochar Modification for Energy Storage in Supercapacitors: A Review
Viral Evolution of Multiple Coronavirus Genomes on Genomic Index Maps
Solution Combustion Synthesis and Energy Transfer in LaMgAl11O19:Tb3+/Sm3+ Tunable Phosphor
Influence of Fe3O4 and CTABr on the Rate of Degradation of Methylene Blue by H2O2
Effect of Sulfurization Temperature on RF Sputtered MoS2 Thin Film
Fatigue Analysis for Fe-34.5Mn-l0Al-0.76C Tidal Turbine Blades using Rainflow Algorithm
Modelling for the Study of Thermoelastic Properties of Nanoparticles
Potato Starch Edible Films as Environmentally Friendly Carriers for Model Drug: In vitro Release Study