An Electrochemical Perspective Assay For Anticancer Activity Of Calotropis Procera Against Glioblastoma Cell Line (LN-18) Using Carbon Nanotubes- Graphene Nano- Conglomerate As A Podium

Seetharamaiah Nalini1,3, Seetharamaiah Nandini1,3, Gurukar Shivappa Suresh1*,Jose Savio Melo2*, Shivayogeeswar E. Neelagund3*, Hunasepalya Nagaiah Naveen Kumar3,Jakkid Sanetuntikul4, Sangaraju Shanmugam4

Adv. Mater. Lett., 2016, 7 (12), pp 1003-1009

DOI: 10.5185/amlett.2016.6395

Publication Date (Web): Oct 12, 2016

Abstract


In this article, we report the pre-screening of anti-cancer effect of Calotropis procera against glioblastoma cell lines (LN-18) by means of electrochemical methods. Soxhlet assisted extraction (SAE) has been employed to extract the polyphenol contents present in the leaf of Calotropis procera. The phytochemical analysis of the extract has been studied and the polyphenol contents were determined using Folin Ciocalteau method. To study the anticancer effects of the aqueous plant extract, a cytosensor (Gr/NT-G/LN-18) was fabricated and its possible mechanism for DNA binding was studied using graphite/ poly(allylamine hydrochloride)/nanotube-graphene composite /polypyrrole /de-oxy ribonucleic acid (Gr/PAH/NT-G/PPy/DNA) modified electrode. The electrochemical characteristics of the proposed Gr/NT-G/LN-18 cytosensor towards the plant extract were evaluated using electrochemical techniques like cyclic voltammetry and differential pulse voltammetry. Scanning electron microscopy (SEM) and energy dispersive analysis of x-ray (EDAX) have been employed to study the physical characterization of the Gr/PAH/NT-G/PPy/DNA modified electrode. These results indicate that the plant extract has an ability to act as an antiglioblastoma against LN-18 cancer cells.

Keywords

Anticancer agents, biological activity, DNA, LN-18 cells, soxhlet assisted extraction.

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