Synthesis, Physico-chemical Characteristics And Cellular Behavior Of Poly (lactic-co-glycolic Acid)/ Gelatin Nanofibrous Scaffolds For Engineering Soft Connective Tissues
1Department of Biomedical Engineering, Tehran Science and Research Branch, Azad University, P.O. Box: 4515/775, Tehran, Iran
2Department of Dental Materials, School of Dentistry, Shahid Beheshti University of Medical Sciences, P.O. Box: 1983963113, Tehran, Iran
3Bioengineering Research Group, Nanotechnology and Advanced Materials Department, Materials and Energy Research Center (MERC), P.O. Box 14155-4777, Tehran, Iran
4Biotechnology Department, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Adv. Mater. Lett., 2016, 7 (2), pp 163-169
Publication Date (Web): Jan 04, 2016
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There have been several attempts to synthesis biodegradable polymeric constructs with adequate porous structures for soft connective tissues. In this study, randomly-oriented PLGA-gelatin nanofibrous scaffolds were synthesized by electrospinning method. We offered an appropriate solvent (2, 2, 2-trifluoroethanol) to dissolve both polymers for achieving a homogenous solution without inducing any toxic effects. The results confirmed the formation of high porous and bead free scaffolds, in which an increase in the injection rate slightly decreased the mechanical, swelling ratio and biodegradation behaviors. The modulus and tensile strength for the scaffolds with the injection rate of 0.2 ml/hr were 0.72 ±0.02 and 2.70 ±0.33, respectively. In addition, the evaluation of cell proliferation demonstrated that L929 fibroblast cells spread well on the scaffolds, indicating that they are able to support cell attachment. A possible chemical bond formation has been also suggested for the blending mixture of PLGA and gelatin molecules.
Nanofibrous, scaffold, electrospinning, tissue engineering, cellular behavior.