Biophysical Study On The Interaction Of Spirooxindole-annulated Thiopyran Derivatives With Bovine Serum Albumin Using Spectroscopic And Docking Methods

Swarup Roy1, Sudipta Ponra2, Raj Kumar Nandi2, K. C. Majumdar2*, Tapan Kumar Das1*

1Department of Biochemistry and Biophysics, University of Kalyani, Kalyani 741235, West Bengal, India

2Department of Chemistry, University of Kalyani, Kalyani 741235, West Bengal, India

Adv. Mater. Lett., 2015, 6 (10), pp 913-919

DOI: 10.5185/amlett.2015.5931

Publication Date (Web): Sep 18, 2015

E-mail: tapankumardas175@gmail.com, kcmklyuniv@gmail.com

Abstract


The study of interaction between spirooxindole-annulated thiopyran derivatives (STP) and bovine serum albumin (BSA) was investigated using multi-spectroscopic and docking method. The intrinsic fluorescence of BSA could effectively quenched by STP through dynamic quenching. The thermodynamic parameters suggested that hydrogen bonds and van der Waals forces played a key role in stabilizing the BSA–STP complexes. According to Forster non radiation energy transfer theory (FRET) the average binding distance (r) between STP and BSA were found to be < 7 nm. Furthermore, UV-visible and circular dichroism results indicated that in presence of STP secondary structure of BSA changed. Theoretical docking study of the interaction of BSA and STP also supported the experimental results.

Keywords

Bovine serum albumin, thiopyran, spectroscopy, CD, docking.

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