Development And Validation Of Biosensing Method For Acetaminophen Drug Monitoring

¹Amity Institute of Nanotechnology, AMITY University, Noida (UP), India

²Amity Institute of Physiotherapy, AMITY University, Noida (UP), India

³Department of Biochemistry, M. D. University, Rohtak 124 001, Haryana, India

⁴Faculty of Pharmaceutical science PGIMS, Rohtak, India

Adv. Mater. Lett., 2015, 6 (3), pp 209-216

DOI: 10.5185/amlett.2015.5675

Publication Date (Web): Feb 22, 2015

Copyright © IAAM-VBRI Press

E-mail: jags_biotech@yahoo.co.in

Abstract

A polyphenol oxidase (PPO) was immobilized by employing magnetic nanoparticles-zinc oxide/zinc hexacyanoferrate (Fe₃O₄NP-ZnO/ZnHCF) hybrid film electrodeposited on the surface of Pt electrode. The surface functionalization of Fe₃O₄NP-ZnO/ZnHCF hybrid film was characterized by cyclic voltammetry (CV), scanning electron microscopy (SEM), atomic force microscopy (AFM) and electrochemical impedance spectroscopy (EIS) before and after immobilization of PPO. The biosensor exhibited optimum response within 4s at pH 7.0 and 35°C and linearity in the range 0.04 to 10000 μM for acetaminophen with a detection limit of 0.04 μM (S/N=3). Accuracy of the proposed sensor was found to be 99%. The use of Fe₃O₄NP/ZnO/ZnOHCF for construction of amperometric acetaminophen biosensor has resulted into relatively rapid response, higher sensitivity, broad linear range, lower detection limit, good reproducibility and long term stability of this biosensor. This sensing interface provides better avenue for the fabrication of various sensor.

Keywords

Drug monitoring, acetaminophen, magnetic nanoparticles, zinc oxide/zinc hexacyanoferrate film, Pt electrode.