1Enzyme Technology Lab, Department of Biosciences, Jamia Millia Islamia, New Delhi 110025, India
2Bioinformatics Institute (BII), a member of A*STAR's Biomedical Sciences Institutes,30 Biopolis Street 07-01 Matrix, Singapore 138671
Adv. Mater. Lett., 2015, 6 (3), pp 194-200
DOI: 10.5185/amlett.2015.5658
Publication Date (Web): Feb 22, 2015
Copyright © IAAM-VBRI Press
E-mail: msardar@jmi.ac.in, dr.meryam@gmail.com
In this paper, we described a simple and single step procedure for the synthesis of horseradish peroxidise enzyme (HRP) capped silver and gold nanoparticles. HRP, a heme-containing enzyme utilises hydrogen peroxide to oxidise a wide variety of organic and inorganic compounds. The biosynthesized nanoparticles were characterized by means of UV-VIS spectroscopy, Dynamic light scattering (DLS), Transmission electron microscopy (TEM), X-ray diffraction (XRD) and Fourier-transform infrared spectroscopy (FTIR). FTIR study confirms the presence of peroxidase enzyme on the nanoparticles. Computational studies reveal that exposed amino acids (viz serine, threonine, arginine and glycine) play key role in reduction and as well as stabilization of nanoparticles. The HRP assisted silver and gold nanoparticles retained its biological activity in the nanoparticles. The study indicates that Peroxidase which is found in almost all the plants can be used for the large scale synthesis of nanoparticles. Moreover additional attraction is the retention of the enzymatic activity on the nanoparticles. In a single step reaction enzyme is catalysing and in doing so it gets immobilized on it. The integration of biomolecules to nanoparticles is a tedious method mainly due to the surface of nanoparticles. Functionalization of noble metal nanoparticles with biomolecules (e.g., protein and DNA) is in demand because such systems possess numerous applications in catalysis, delivery, therapy, and imaging, sensing and controlling the structure of biomolecules. Computational study highlighted the amino acids which are interacting with the metal ions, thus synthetic peptides can also be designed to synthesize the metal nanoparticles.
Advancing Materials towards Climate Neutrality by 2050
Efficiency of Nanomaterials for Electrochemical Diagnostics based Point-of-Care Detection of Non-Invasive Oral Cancer Biomarkers
Hybrid Membrane based on Polymer-doped Phosposilicate and their Characterization
A DFT Study of Interaction of (CdSe)3 Quantum Dots with Nucleobases
Low Resistance and High Electromigration Lifetime of Cu-To-Cu Joints Using (111)-Oriented Nanotwinned Copper
Electrochemical Hydrogen Evolution Reaction Triggered by Co2xMo1-xS2 (x = 0. 0.05 and 0.1) in Acidic Medium
Effect of La Dopant in Modified Bismuth Ferrite Ceramics
Biogenic Capped Silver Nanoparticles in Lablal Purpureus Pod Extract Exhibit Selective Antibacterial and Synergistic Anticancer Activity
Transforming Paradigm of Artificial Intelligence for Scalable Clinical Healthcare
A Review on Harnessing Nanomaterials as Promising Materials Interface
A New Epidemic Spreading Model to Predict the Spread and Accumulation of Corona Virus (COVID-19) Positive Cases as a Function of Time
Green Synthesis of ZnO and Ag-ZnO Nanoparticles using Macrotyloma Uniflorum: Evaluation of Antibacterial Activity
Feature Behavior of Resistivity in Bi Foils Obtained by a Melt Spinning Method
Thermomagnetic Properties of Metal and Metal-oxide Nanoparticles
Effect on Structural, Electrical and Temperature Sensing behavior of Neodymium Doped Bismuth Ferrite
Novel Green Chemistry Synthesis of Nano-Hydroxyapatite using Soya Milk as a Natural Stabiliser
Copyright ©2010-2021 VBRI Press
Full Article