1Centro de Nanociencia y Nanotecnologia, Universidad de las Fuerzas Armadas -ESPE, Av. Gral. Rumiñahui s/n, Sangolqui, P.O. BOX 171-5-231B, Ecuador
2Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Departamento de Farmacología. C.P. 07360, Mexico City, Mexico
Adv. Mater. Lett., 2015, 6 (2), pp 127-132
Publication Date (Web): Feb 08, 2015
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Silver nanoparticles (AgNPs) have been synthesized via the green pomosynthetic procedure, using Passiflora tripartita var. mollissima fruit pigments as both the reducing and stabilizing agents. UV–Vis Spectroscopy, Dynamic Light Scattering, Transmission Electron Microscopy with Selected Area Electron Diffraction and Powder X-Ray Diffraction are used to completely characterize the AgNPs. The prepared AgNPs are found to be mostly spherical shapes with an average diameter of 49.7 ± 24.6 nm at room temperature. XRD analysis revealed the face-centered cubic geometry of AgNPs whereas Infrared spectrum and cyclic voltammetry measurements hypothesize the possible biomolecules (flavonoid C & O-glycosides) responsible for stabilization of the AgNPs. Synthesized AgNPs shows significant antioxidant efficacy (67%, 0.15 mM) against 1, 1-diphenyl-2- picrylhydrazyl. The AgNPs (0.01 – 20 μΜ) did not affect cell proliferation of the human cancer cell lines A -549 and HeLa, from lung and cervix, respectively. The use of environmentally benign, cost-effective and renewable materials like P. tripartita extract offers numerous benefits of eco-friendliness and compatibility for potential future pharmaceutical and biomedical applications.
Silver nanoparticles, Passiflora tripartita, TEM, XRD, antioxidant, cancer cells.