Amperometric Immuno-sensor For Detection Of Toxin Aflatoxin B1 Based On Polyaniline Probe Modified With Mc-IgGs-a-AFB1 Antibodies

Keisham Radhapyari1,2, Raju Khan1*

1Analytical Chemistry Division, CSIR-North East Institute of Science & Technology, Jorhat 785006, Assam, India

2Central Ground Water Board, North Eastern Region, Ministry of Water Resources,   Government of India, Tarun Nagar, Guwahati 781005, Assam, India

Adv. Mater. Lett., 2014, 5 (8), pp 435-440

DOI: 10.5185/amlett.2014.5562

Publication Date (Web): Jul 16, 2014

E-mail: khan.raju@gmail.com

Abstract


An amperometric sensor for detection of toxin aflatoxin B1 from aspergillus flavus based on conducting polyaniline probe using monoclonal anti-aflatoxin B1 (Mc-IgGs-a-AFB1) antibodies after activation with 3% Bovine Serum Albumin (BSA) through electrochemical polymerization has been proposed. The electrode was fabricated by immobilizing Mc-IgGs-a-AFB1 antibodies molecules onto electrode surface and characterized by cyclic voltammetry (CV), scanning electron microscopy (SEM) and fourier transform infrared spectroscopic (FT-IR) etc. The proposed amperometric immune-sensor has demonstrated excellent electro-analytical properties relative to Aflatoxin B1 in a linear range from 0.20 to 1.30 AngmL-1 with a relatively low detection limit of 0.059 AngmL-1. The present study will help in improving for quantitative determination of mycotoxins in food samples may provide significant improvements in quality control of food safety through a simple, rapid, and sensitive testing system for agricultural products monitoring.

Keywords

Immuno-sensor, polyaniline, bovine serum albumin, environmental toxins, aflatoxin B1, electrochemical impedance spectroscopy

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