Study Of In Vitro Toxicity Of Glucose Capped Gold nanoparticles In Malignant And Normal Cell Lines

Harminder Kaur1, Geetanjali Pujari1, Asitikantha Sarma1, Yogendra Kumar Mishra2, Mi Kyung Jin3, Bikesh K. Nirala4, Nivedita K Gohil4, Rainer Adelung2, Devesh Kumar Avasthi1*

1 Inter University Accelerator Center, Aruna Asaf Ali Marg, New Delhi, 110067, India

2Functional Nanomaterials, Institute for Materials Science, University of Kiel, Kaiserstr. 2, Kiel, 24143, Germany

3Division of Nephrology and Hypertension, University of Kiel, Schittenhelmstr. 12, Kiel, 24105, Germany

4Centre for Biomedical Engineering, Indian Institute of Technology, New Delhi, 110016, India

Adv. Mater. Lett., 2013, 4 (12), pp 888-894

DOI: 10.5185/amlett.2013.5484

Publication Date (Web): Nov 24, 2013

E-mail: dka@iuac.res.in

Abstract


Gold nanoparticles (AuNPs) are potential candidates for targeted drug delivery, imaging and early detection of cancer cells due to their ability to bind with cancer cells. To ensure their safe use in various possible biomedical applications, it is essential to examine the cytotoxicity and biocompatibility of AuNPs before use. The present work aims to study the cytotoxicity of glucose capped gold nanoparticles (Glu-AuNPs) in several cell lines (HeLa, A549, Jurkat, L929 and HUVEC). The synthesized Glu-AuNPs, using β-D glucose as reducing as well as capping agent, were characterized by SPR and TEM/EDAX analysis. Internalization of Glu-AuNPs in cells was studied by cross sectional TEM imaging. The cytotoxicity of Glu-AuNPs was evaluated by means of colony formation and MTT assays. The present study reveals that Glu-AuNPs (7±2 nm diameter) are non-toxic to the above-mentioned five cell lines, which are cancerous cell lines except HUVEC. Therefore Glu-AuNPs (around 7nm) can be explored for various bio-medical applications and can be of importance for therapeutic applications as evident from enhancement in radiosensitization from our previous work.

Keywords

Glucose capped gold nanoparticles, Glu-AuNPs, cytotoxicity, colony forming assay, MTT assay.

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