Shandong Provincial Key Laboratory of Fluorine Chemistry and Chemical Materials, School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250 022, China
Adv. Mater. Lett., 2010, 1 (1), pp 34-39
DOI: 10.5185/amlett.2010.3104
Publication Date (Web): Apr 08, 2012
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Nano-scale hydroxyapatite (HAp) was prepared by combining co-precipitation with microemulsion method, which exhibited strong adsorption for DNA due to its excellent biocompatibility and particular adsorbability. DNA and HAp could be modified onto glassy carbon electrode (GCE) by the simple and convenient “tip-coating” method. Cyclic voltammetry was used to investigate the interaction of DNA immobilized on the HAp film with vitamin B12 (VB12). The existence of DNA led to the decrease of reduction current of VB12. Both the electron transfer coefficient (α) and the standard rate constant (ks) were different obtained on GCE and dsDNA/HAp/GCE, which indicated the formation of an electrochemical inactive super molecular complex DNA-nVB12. The equilibrium constant of this complex was calculated to be 5.35 × 105 mol·L-1 and the binding number between DNA and VB12 of the complex were determined to be one.
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