Efficient siRNA delivery using osmotically active and biodegradable poly(ester amine)
1Department of Molecular Genetics, School of Dentistry, Seoul National University, Seoul 08826 Republic of Korea
2Y. B. Chavan College of Pharmacy, Dr. Rafiq Zakaria Campus, Aurangabad, MH State, India
3Dental Research Institute, School of Dentistry, Seoul National University, Seoul 03080, Republic of Korea
Adv. Mater. Lett., 2018, 9 (8), pp 590-593
Publication Date (Web): Jun 14, 2018
Copyright © IAAM-VBRI Press
Biodegradable and hyperbranched poly(ester amine) (PEA) was prepared by reaction of glycerol dimethacrylate (GDM) with low molecular weight polyethylenimine (LMW-PEI) by Michael addition reaction. This novel gene carrier showed excellent physicochemical properties and relatively low cytotoxicity compared with PEI 25K. It showed excellent transfection efficiency and siRNA delivery. The higher silencing efficiency of PEAs could be attributed to the synergistic effect arising from hyperosmotic glycerol and proton sponge active PEI residues in the PEA backbone.
Poly (ester amine), hyperosmotic effect, proton sponge effect, siRNA delivery.