A sensitive electrochemical aptasensor based on single wall carbon nanotube modified screen printed electrode for detection of Escherichia coli O157:H7

M.R. Housaindokht1*, A.Verdian2, E. Sheikhzadeh3, P. Pordeli4, Z. Rouhbakhsh Zaeri3, F. Janati-Fard3, M. Nosrati3 M. Mashreghi4, A. R.Haghparast1, A. Nakhaeipour3, A. A. Esmaeili1, S. Solimani5

1Research and Technology Center for Biomolecules, Ferdowsi University of Mashhad, Mashhad, Iran

2Department of Food Nanotechnology, Research Institute of Food Science and Technology (RIFST), Mashhad, Iran

3Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran

4Department of Biology, School of Sciences, Ferdowsi University of Mashhad, Mashhad, Iran

5Department of Pathobiology, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran 

Adv. Mater. Lett., 2018, 9 (5), pp 369-374

DOI: 10.5185/amlett.2018.1701

Publication Date (Web): May 17, 2018

E-mail: elhamsheikhzadeh@yahoo.com

Abstract


Escherichia coli O157:H7 (E.coli O157:H7), is an extremely infectious and potentially fatal water and food-borne pathogen, responsible for numerous hospitalisations and deaths all over the world. Herein we reported the development of simple and label-free electrochemical aptasensor based on single wall carbon nanotube (SWCNT) modified screen printed electrode (SPE) to detect E.coli O157:H7 bacteria. The electrochemical probe of methylene blue (MB) was used to investigate the interactions between aptamer and E.coli O157:H7. The resulting MB/Apt/SWNT/SPE layer exhibited good current response to detect E.coli O157:H7. The aptasensor detect E.coli O157:H7 in the concentration range of 1.7×101 - 1.1×107 CFU mL-1 and limit of quantification of 1.7×101 CFU mL-1. The proposed aptasensor is rapid, convenient and low-cost for effective sensing of E.coli O157:H7 with high selectivity over interference bacteria. The suitability of the aptasensor for real sample measurements was investigated by recovery studies in tap water sample. 

Keywords

Escherichia coli O157:H7, aptamer, electrochemical aptasensor, label-free detection.

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