Impedimetric analysis of interaction between DNA and phytochemical constituents’ of Calotropis procera for pre-clinical evaluation

Seetharamaiah Nalini1, 2, Seetharamaiah Nandini1, 2, Shivayogeeswar E. Neelagund2*,  Jose Savio Melo3*, Gurukar Shivappa Suresh1*

1Department of Chemistry and Research Centre, N.M.K.R.V. College for Women, Jayanagar, Bangalore 560 011, India

2Department of PG Studies and Research in Biochemistry, Jnana Sahyadri, Kuvempu University,

Shankaraghatta 577 451, Shivamogga, Karnataka, India

3Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, India

Adv. Mater. Lett., 2017, 8 (11), pp 1020-1028

DOI: 10.5185/amlett.2017.1554

Publication Date (Web): Aug 05, 2017

E-mail: sureshssmrv@yahoo.co.in, jsmelo@barc.gov.in, neelgund@gmail.com

Abstract


In this report, extractions of the Calotropis procera polyphenols have been carried out in conventional soxhlet assisted extraction (SAE) and it is compared with microwave assisted extraction (MAE). As an extension of our previous work reported, we attempted to study the electrochemical interaction of the aqueous plant extract with DNA modified electrode Gr/PAH/NT-G/PPy/DNA. The binding constant Kf determined using UV-Vis spectroscopy was found to be 4.91×10-1 and 4.55×10-1 µmol dm-3 for soxhlet assisted plant extract (SAPE) and microwave assisted plant extract (MAPE) respectively. This was consistent with the electrochemical determination of Kf 4.31×10-6 and 4.71×10-4 µmol dm-3 for SAPE and MAPE respectively. Further the surface coverage area (Γs) of the electrode fabricated using DNA was calculated using Chronocoulometry for the plant extracts. The linear range of the aqueous extract on the Gr/PAH/NT-G/PPy/DNA modified electrode was found to be from 1-250 µmol dm-3, with a limit of detection of 0.22 and 0.29 µmol dm-3 mL-1 for SAPE and MAPE respectively. The limit of quantification was found to be 0.68 and 0.88 µmol dm-3 mL-1 for SAPE and MAPE respectively. As a result Calotropis procera can be studied further to applied for anticancer activity. Moreover, electrochemical methods can be a promising technique in drug analysis.

Keywords

Impedimetric detection, soxhlet assisted extraction, microwave assisted extraction, chronocoulometry, spectrophotometry.

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